Effect of Plant Growth Regulators on Callus Induction in Piper betle L. var. Nigra
Pengaruh Berbagai Jenis ZPT terhadap Induksi Kalus Piper betle L. var. Nigra
DOI:
https://doi.org/10.53342/pharmasci.v8i1.302Keywords:
Callus induction, Piper betle L. var. Nigra, Plant Growth RegulatorsAbstract
Callus culture is one of the plant tissue culture techniques that used to study aspects of plant nutrition, somatic embryogenesis, cell suspension culture, secondary metabolite production, and genetic transformation. Callus induction was carried by adding growth regulators. Black betel (Piper betle L. var. nigra) is a medicinal plant that has the potential to produce secondary metabolites. The aim of this study was to obtain the best formula for callus induction of P. betle L. var. nigra. The single growth regulator used is 2,4-Dichlorophenoxyacetic acid (2,4-D), Benzyl Amino Purine (BAP), Indole Butyric Acid (IBA), Indole Acetic Acid (IAA), and Naphthalene Acetic Acid (NAA) with variations the concentration used is 0; 0.5; 1; 1.5; 2; 2.5 mg/L. Each treatment consisted of 6 replicates grown using Murashige and Skoog (MS) medium in vitro for eight weeks. The results showed that the 1.5 mg/L 2,4-D treatment produced the highest fresh weight (1389.5 mg) and dry weight (55.7 mg). Callus P. betle L. var. Nigra in various treatments showed various textures such as compact and friable with callus colors such as white, greenish-white, brownish-white, yellowish-green, greenish-yellow, brownish-yellow, greenish-brown, brown, and gray.
References
Jadhav SB, Shah N, Rathi A, Rathi V, Rathi A. Serratiopeptidase: Insights into the therapeutic applications. Biotechnology Reports. 2020: 00544.
Simpson MG. Plant systematics. United States. Elsevier Academic Press; 2006.
Munawaroh E. The diversity and conservation of Piper (Piperaceae) in Bukit Barisan Selatan National Park, Lampung Province. Media Konservasi. 2017;22(2):118-28.
Junairiah, Ni’matuzahroh, Zuraidassanaaz NI, Sulistyorini L. Isolation and identification of secondary metabolites of black betel (Piper betle L. var. Nigra). Jurnal Kimia Riset. 2018;3(2):131-38.
Sari YP, Kusumawati E, Saleh C, Kustiawan W, Sukartingsih S. Effect of sucrose and plant growth regulators on callogenesis and preliminary secondary metabolic of different explant Myrmecodia tuberosa. Nusantara Bioscience. 2018;10(3):183-92.
Sharma PR, Patil AV, Patil DA. Effect of culture media and growth hormones on callus induction in Crataeva tapia L. Int. J. Pharm. Res. 2017;9:70-6.
Malik SI, Rashid H, Yasmin TA, Minhas NM. Effect of 2, 4-dichlorophenoxyacetic acid on callus induction from mature wheat (Triticum aestivum L.) seeds. Int. J. Agric. Biol. 2003;6:156-9.
Budisantoso I, Amalia N, Kamsinah K. In vitro callus induction from leaf explants of Vanda sp stimulated by 2, 4-D. Biosaintifika: Journal of Biology & Biology Education. 2017;9(3):492-7.
Zulkarnain Z, Neliyati N, Lizawati L. Callus proliferation from immature leaf explants of durian (Durio zibethinus Murr. cv. Selat) with the addition of Picloram and BAP. Jurnal Hortikultura Indonesia. 2013;4(3):107-14.
Thirupathy S, Sisubalan N, Ghouse BM. Callus induction from a wild medicinal plant Tephrosia hookeriana (weight and arn). International Journal of Recent Scientific Research. 2014;5(6):1027-30.
Gauchan DP, Dhakal A, Sharma N, Bhandari S, Maskey E, Shrestha N, Gautam R, Giri S, Gurung S. Regenerative callus induction and biochemical analysis of Stevia rebaudiana Bertoni. Journal of Advanced Laboratory Research in Biology. 2014;5(3):41-5.
Purwianingsih W, Hamdiyati Y. Metode elisitasi menggunakan ragi Sacharomyces cerevisae untuk meningkatkan kandungan bioaktif kuinon kalus Morinda cirtrifolia L. (mengkudu). Universitas Pendidikan Indonesia. Bandung. 2007;1-14.
Elangomathavan R, Kalaivanan P, Hariharan P, Beaulah SN. High efficient protocol for callus induction and regeneration of a medicinal plant Orthosiphon stamineus. International Journal of Advanced Research in Biological Sciences. 2017;4(1):113-22.
Nasution NH, Nasution IW. The effect of plant growth regulators on callus induction of mangosteen (Garcinia mangostana L.). InIOP Conference Series: Earth and Environmental Science 2019;305(1):012049.
Lin J, Wu L, Liang J, Wang J. Effect of different plant growth regulators on callus induction in Catalpa bungei. African Journal of Agricultural Research. 2010;5(19):2694-704.
Ivan GR, Carlos RM, Tatiana CD, Marsen GP, Norma A. Compact callus cultures and evaluation of the antioxidant activity of Hovenia dulcis Thunb.(Rhamnaceae) under in vivo and in vitro culture conditions. Journal of Medicinal Plants Research. 2015;9(1):8-15.
Ahmed AB, Pallela R, Rao AS, Rao MV, Taha RM. Optimized conditions for callus induction, plant regeneration and alkaloids accumulation in stem and shoot tip explants of Phyla nodiflora. Spanish Journal of Agricultural Research. 2011;9(4):1262-70.
