Acute Toxicity Test of Red Bajakah Bark Decoction (Uncaria nervosa Elmer) in Female Mice

Uji Toksisitas Akut Rebusan Kulit Batang Bajakah Merah (Uncaria nervosa Elmer) Pada Mencit Betina

Abstract

Uncaria nervosa Elmer is a type of bajakah plant that contains many compounds that have potential in the pharmaceutical field, which one is anticancer. In this study, acute oral toxicity testing was carried out with the aim of determining the LD50 value of administering a decoction of  bajakah skin (Uncaria nervosa Elmer). Twenty female mice were used as experimental animals and divided into 4 groups, consisting of 3 test dose groups and 1 control group. The doses used are 300, 2000, and 5000 mg/kg BW. A single dose was administered orally on day 0 and observations were carried out until day 14, then the number of test animals that died in each group, clinical symptoms, liver organ index, and liver cell tissue of mice were calculated. The results of the secondary metabolite test showed that there were flavonoids, alkaloids and phenolics contained. The results of the research on the toxicity test showed that the LD50 value was LD50 of Bajakah boiled orally, which was 5774.3 mg/kgBW and was classified in the non-toxic group (>5000 mg/kg).